Beyoncé gave her father his walking papers, after two decades of him running the show and calling all the shots she finally sets him free…

The multiplatinum-selling singer announced last night that she and dad Matthew Knowles parted ways “on a business level,” said publicist Yvette Noel-Schure.

The father-daughter relationship has been strained since Matthew and Tina Knowles divorced in 2009, after 29 years of marriage.

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